DEVELOPMENT OF A TOPICAL PLATFORM FOR THE DELIVERY OF NATURAL BIOACTIVE COMPOUNDS AND STAPHYLOCOCCUS AUREUS PHAGES FOR DERMAL APPLICATIONS - CY Cergy Paris Université Accéder directement au contenu
Communication Dans Un Congrès Année : 2024

DEVELOPMENT OF A TOPICAL PLATFORM FOR THE DELIVERY OF NATURAL BIOACTIVE COMPOUNDS AND STAPHYLOCOCCUS AUREUS PHAGES FOR DERMAL APPLICATIONS

Résumé

Perturbations of the skin functions are related to many factors, such as high oxidative stress and microbial dysbiosis that, in some cases, may lead to infections and formation of biofilms on the skin surface. Combination therapy based on antioxidants and antibacterials may be a key strategy to consider for the treatment of dermal pathologies. Curcumin (CUR) is a multi-targeting pharmacologically active compound known, among others, for its antioxidant properties. However, the hydrophobic nature and the unstable structure of curcumin limits its capacity to penetrate the skin. A strategy to deliver curcumin is to encapsulate it in nanostructured lipid carriers (NLCs), to preserve its antioxidant properties. Lytic phages are viruses that specifically target and infect bacteria, reproducing within the bacterial cells and ultimately causing their destruction. The use of phages is particularly relevant in the context of the increasing prevalence of multidrug-resistant pathogens, where conventional antibiotics may be less effective. Herein, we propose in this work a bi-functionalized platform for the delivery of curcumin encapsulated in nanostructured lipid carriers (Curcumin-loaded NLCs) and Staphylococcus aureus phages, both entrapped in a hydrogel protective matrix for a topical application. A suspension of NLCs (mainly 85 nm in size) was produced by hot homogenization method and characterized by Dynamic Light Scattering (DLS) and Z-Potential. Entrapment efficiency of CUR in NLCs were assessed by spectrophotometry and more than 50 % of CUR has been encapsulated. A stock of 1010 UFP/mL of phages GRCS was prepared with the double-layer agar plating method. Infectivity studies reveal that the presence of Curcumin-loaded NLCs does not compromise the lytic activity of Staphylococcus aureus phages with <1 log titer reductions. Release of phages was observed in Carbopol hydrogels, with >108 PFU/mL release within 24 h. Finally, preliminary cell viability studies on human fibroblasts (BJ) demonstrate that Curcumin-loaded NLCs and phages GRCS showed no cytotoxicity at concentrations below 0.54 mg/mL (containing 10 µM of curcumin) and 109 PFU/mL respectively. First results showed that the combination of Curcumin-loaded NLCs and S. aureus phages represents a promising strategy for the treatment of skin associated diseases and cutaneous wound. Acknowledgements: This work was funded by CY Initiative of Excellence (grant "Investissements d'Avenir" ANR-16-IDEX-0008 ») and by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe (Grant PID2021-128213OB-I00).
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Dates et versions

hal-04448773 , version 1 (09-02-2024)

Identifiants

  • HAL Id : hal-04448773 , version 1

Citer

Carole Armal, Rosa Calderon-Jacinto, Kandas Traore, Jose Carlos Rodríguez Hernández, Ana Vallés Lluch, et al.. DEVELOPMENT OF A TOPICAL PLATFORM FOR THE DELIVERY OF NATURAL BIOACTIVE COMPOUNDS AND STAPHYLOCOCCUS AUREUS PHAGES FOR DERMAL APPLICATIONS. Journées BIOMAT & MatSAN 2024, Jan 2024, Super-Besse, France. ⟨hal-04448773⟩
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