TOPICAL DERMAL PLATFORM FOR THE CO-DELIVERY OF CURCUMIN AND STAPHYLOCOCCUS AUREUS PHAGES - CY Cergy Paris Université Accéder directement au contenu
Poster De Conférence Année : 2023

TOPICAL DERMAL PLATFORM FOR THE CO-DELIVERY OF CURCUMIN AND STAPHYLOCOCCUS AUREUS PHAGES

Résumé

The clinical evolution of some skin pathologies can be compromised by several factors including the presence of strong oxidative stress, microbial dysbiosis and infectious biofilms. The increasing prevalence of multidrug resistant pathogens and the high tolerance of biofilms to antibiotics make it necessary to search for therapeutic alternatives. In this regard, the use of lytic phages is a promising strategy to fight against infectious bacterial biofilms. In this work we propose a delivery platform containing Curcumin, a polyphenol compound from the rhizome of Curcuma longa, with anti-inflammatory and antioxidant activities, and Staphylococcus aureus phages as antimicrobial and anti-biofilm agents. The hydrophobic character and instability of Curcumin makes necessary its entrapment on specific vehicles. Nanostructured lipid carriers (NLCs), with excellent biocompatibility, represent a suitable option for the topical route. Both Curcumin-loaded NLCs and Staphylococcus aureus phages were entrapped into a hydrogel matrix that also provides moisturisation and helps for a topical application. Components of the delivery platform were prepared and studied as follows: Curcumin-loaded NLCs were produced by hot homogenization method and characterized by Dynamic Light Scattering (DLS) and Z-Potential, showing the feasibility in the production of stable, negatively charged Curcumin-loaded NLCs of 80 nm, (curcumin encapsulation efficiency of 85%). Staphylococcus aureus phages suspension of 1010 UFP/mL were produced and tittered by traditional top agar overlay method. Infectivity studies showed that the presence of Curcumin-loaded NLCs did not affect the lytic activity of Staphylococcus aureus phages. Preliminary cell viability studies of both Curcumin-loaded NLCs and Staphylococcus aureus phages were assessed on human Fibroblasts (BJ). First results show that developed NLCs were found to be non-toxic at concentrations below 0.54 mg/mL (containing 10 µM of curcumin). Preliminary results point that the combination strategy can represent a suitable option as topical skin treatment. This work was funded by CY Initiative of Excellence (grant "Investissements d'Avenir" ANR-16-IDEX-0008 ») and by MCIN/AEI/10.13039/501100011033 and by ERDF A way of making Europe (Grant PID2021-128213OB-I00).
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Dates et versions

hal-04448746 , version 1 (09-02-2024)

Identifiants

  • HAL Id : hal-04448746 , version 1

Citer

Carole Armal, Rosa Calderon-Jacinto, Kandas Traore, Jose Carlos Rodríguez Hernández, Ana Vallés Lluch, et al.. TOPICAL DERMAL PLATFORM FOR THE CO-DELIVERY OF CURCUMIN AND STAPHYLOCOCCUS AUREUS PHAGES. Phages in Lyon 2023, Nov 2023, Lyon, France. ⟨hal-04448746⟩
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