Mimicking the dynamics of the cell microenvironment via electromechanically active and microporous polyHIPE-PEDOT scaffolds - Equipe de recherche sur les relations matrice extracellullaires-cellules Accéder directement au contenu
Poster De Conférence Année : 2023

Mimicking the dynamics of the cell microenvironment via electromechanically active and microporous polyHIPE-PEDOT scaffolds

Résumé

In vivo, cells are surrounded by an extracellular matrix (ECM). Signals from the ECM such as biochemical or biophysical cues regulate cell behavior significantly. Although, 3D cell cultures offer biologically superior structures, there is still a lack in the transmission of dynamic mechanical signals that exist in the microenvironment of cells in vivo. The aim of this work is to develop a 4D microporous and electroactive scaffold as an innovative cell culture platform that enables the in situ electromechanical stimulation of cells and monitoring of the cell behavior. For this purpose, a 3D microporous scaffold is obtained from the polymerization of a high internal phase emulsion template. This so called polyHIPE is characterized by a high interconnectivity and a suitable porosity for a rapid cell colonization. In a further step the polyHIPE-scaffold is functionalized with a conducting polymer, the poly(3,4-ethylenedioxthiophene) (PEDOT). The functionalization leads to a 4D electroactive polyHIPE-PEDOT scaffold with stimuli-responsive properties such as changes in shape, morphology and pore size under external stimulation. Our results show that the stimulation process is reversible, stable and generates volume variations up to 10% on the scaffolds. Furthermore, the polyHIPE-PEDOT scaffolds support cell adhesion, spreading, migration and the cell viability is not affected by 5h of electromechanical stimulation. Main objective Participation to this conference was partially supported by I-MAT federation (FD 4122), INEX-CY Initiative (3DSTIM-2019-054-C01A0) and RESPORE IDF (4D E-BIOCELL).
Fichier principal
Vignette du fichier
Poster_EuroEAP 2023.pdf (1.82 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04400326 , version 1 (21-01-2024)

Identifiants

  • HAL Id : hal-04400326 , version 1

Citer

Franziska Hahn, Ana Ferrández Montero, Cedric Vancaeyzeele, Rémy Agniel, Johanne Leroy-Dudal, et al.. Mimicking the dynamics of the cell microenvironment via electromechanically active and microporous polyHIPE-PEDOT scaffolds. International conference on Electromechanically Active Polymer (EAP) transducers & artificial muscles (EUROEAP), Jun 2023, Bristol, UK, United Kingdom. ⟨hal-04400326⟩
11 Consultations
7 Téléchargements

Partager

Gmail Mastodon Facebook X LinkedIn More